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antibody against itch  (Proteintech)


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    Proteintech antibody against itch
    Antibody Against Itch, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+itch/ITCH+Antibody/10__1096_slash_fj__202502366r-60-19-22
    Average 93 stars, based on 28 article reviews
    antibody against itch - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: E3 ubiquitin ligase ITCH-mediated proteasomal degradation of WBP2 sensitizes breast cancer cells to chemotherapy through restraining AMOTL2/c-JUN axis.
    Article Snippet: Our study had demonstrated that WW domain-binding protein 2 (WBP2) conferred chemoresistance in breast cancer (BC).. However, the underlying mechanism remains unclear.. Herein, a decreased expression of itchy E3 ubiquitin protein ligase (ITCH) was observed in drug-resistant BC tissues which negatively regulated the expression of WBP2.

    Article Title: Identification and validation of CDC20 and ITCH as ubiquitination related biomarker in idiopathic pulmonary fibrosis
    Article Snippet: The antibodies used included anti-ITCH (Proteintech, China), anti-CDC20 (Proteintech, China), anti-α-SMA (Proteintech, China), and anti-β-ACTIN (Proteintech, China).

    Article Title: Identification and validation of CDC20 and ITCH as ubiquitination related biomarker in idiopathic pulmonary fibrosis.
    Article Snippet: The antibodies used included anti-ITCH (Proteintech, China), anti-CDC20 (Proteintech, China), anti-α-SMA (Proteintech, China), and anti-β-ACTIN (Proteintech, China).

    Article Title: JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling
    Article Snippet: The following antibodies were used: Anti-β-actin, anti-α-tubulin, anti-GAPDH, anti-HA (1;1000, Beyotime, Jiangsu, China); anti-HER2, anti-HER3, anti-p-p38(Thr180/Thr182); anti-Ub (1:1000, CST, USA); anti-JWA (1:100, Laboratory-made); anti-GATA-1, anti-NEDD4, anti-CBL, anti-SMURF1, anti-ITCH, anti-HER1, and anti-HER4 (1:1000, proteintech, China).

    Article Title: ECHDC2 inhibits the proliferation of gastric cancer cells by binding with NEDD4 to degrade MCCC2 and reduce aerobic glycolysis
    Article Snippet: The antibodies used were as follows: anti-MCCC2 (12117-1-AP, Proteintech, China); anti-ECHDC2 (bs-13049R, Bioss, China); anti-GLUT1 (21829-1-AP, Proteintech, China); anti-PKM2 (15822-1-AP, Proteintech, China); anti-SDHA (14865-1-AP, Proteintech, China); anti-G6PD (ab993, Abcam, UK); anti-c-Myc (10828-1-AP, Proteintech, China); anti-p-AKT (66444-1-lg, Proteintech, China); anti-P38 MAPK (14064-1-AP, Proteintech, China); anti-ubiquitin (10201-2-AP, Proteintech, China); anti-NEDD4 (21698-1-AP, Proteintech, China); anti-ITCH (20920-1-AP, Proteintech, China); anti-CBL (25818-1-AP, Proteintech, China); anti-GAPDH (10494-1-AP, Proteintech, China).

    Ubiquitin Proteomics:

    Article Title: Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.
    Article Snippet: Spinal muscular atrophy (SMA), a degenerative motor neuron disease and a leading cause of infant mortality, is caused by loss of functional survival motor neuron (SMN) protein due to SMN1 gene mutation.. Here, using mouse and cell models for behavioral and histological studies, we found that SENP2 (SUMO/sentrin-specific protease 2)-deficient mice developed a notable SMA-like pathology phenotype with significantly decreased muscle fibers and motor neurons.. At the molecular level, SENP2 deficiency in mice did not affect transcription but decreased SMN protein levels by promoting the SUMOylation of SMN.



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    itch  (Santa Cruz Biotechnology)
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    ( A ) Immunoblot analysis of <t>ITCH</t> levels in HEK293 cells transduced with lentiviral shRNAs targeting USP11, showing that USP11 positively regulate ITCH levels ( n = 3). ( B ) Immunoblot analysis of ITCH levels in HEK293 cells treated with Sp-1 or MTX, the small molecule inhibitor of USP10 or USP11, respectively. The inhibition of USP11, but not USP10, led to a decrease in ITCH protein level ( n = 3). ( C and D ) Reciprocal coimmunoprecipitations between USP11 and ITCH, followed by immunoblot analysis. ( E ) A direct binding analysis using purified proteins showed that Flag-ITCH pulled GST-USP11 but not the GST control protein. ( F ) IF analysis of endogenous ITCH and USP11 in HEK293 cells revealed a colocalization between the two native proteins in the cytoplasm. Scale bar, 10 μm. ( G ) The PLA analysis of the interaction between endogenous ITCH and USP11 in HeLa cells using a rabbit anti-USP11 antibody paired with a <t>mouse</t> <t>anti-ITCH</t> antibody. Negative controls included nonspecific mouse IgG or rabbit IgG paired with the USP11 or ITCH antibody, respectively ( n = 30 cells). IP, immunoprecipitation.
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    Image Search Results


    ( A ) Immunoblot analysis of ITCH levels in HEK293 cells transduced with lentiviral shRNAs targeting USP11, showing that USP11 positively regulate ITCH levels ( n = 3). ( B ) Immunoblot analysis of ITCH levels in HEK293 cells treated with Sp-1 or MTX, the small molecule inhibitor of USP10 or USP11, respectively. The inhibition of USP11, but not USP10, led to a decrease in ITCH protein level ( n = 3). ( C and D ) Reciprocal coimmunoprecipitations between USP11 and ITCH, followed by immunoblot analysis. ( E ) A direct binding analysis using purified proteins showed that Flag-ITCH pulled GST-USP11 but not the GST control protein. ( F ) IF analysis of endogenous ITCH and USP11 in HEK293 cells revealed a colocalization between the two native proteins in the cytoplasm. Scale bar, 10 μm. ( G ) The PLA analysis of the interaction between endogenous ITCH and USP11 in HeLa cells using a rabbit anti-USP11 antibody paired with a mouse anti-ITCH antibody. Negative controls included nonspecific mouse IgG or rabbit IgG paired with the USP11 or ITCH antibody, respectively ( n = 30 cells). IP, immunoprecipitation.

    Journal: Science Advances

    Article Title: ITCH regulates Golgi integrity and proteotoxicity in neurodegeneration

    doi: 10.1126/sciadv.ado4330

    Figure Lengend Snippet: ( A ) Immunoblot analysis of ITCH levels in HEK293 cells transduced with lentiviral shRNAs targeting USP11, showing that USP11 positively regulate ITCH levels ( n = 3). ( B ) Immunoblot analysis of ITCH levels in HEK293 cells treated with Sp-1 or MTX, the small molecule inhibitor of USP10 or USP11, respectively. The inhibition of USP11, but not USP10, led to a decrease in ITCH protein level ( n = 3). ( C and D ) Reciprocal coimmunoprecipitations between USP11 and ITCH, followed by immunoblot analysis. ( E ) A direct binding analysis using purified proteins showed that Flag-ITCH pulled GST-USP11 but not the GST control protein. ( F ) IF analysis of endogenous ITCH and USP11 in HEK293 cells revealed a colocalization between the two native proteins in the cytoplasm. Scale bar, 10 μm. ( G ) The PLA analysis of the interaction between endogenous ITCH and USP11 in HeLa cells using a rabbit anti-USP11 antibody paired with a mouse anti-ITCH antibody. Negative controls included nonspecific mouse IgG or rabbit IgG paired with the USP11 or ITCH antibody, respectively ( n = 30 cells). IP, immunoprecipitation.

    Article Snippet: The following antibodies were used for WB, IF, and IHC with the indicated dilutions: Flag (Sigma-Aldrich, F1804, 1:200 for IF); Flag (Sigma-Aldrich, F3165, 1:1000 for WB); Flag (Cell Signaling Technology, 14793S, 1:400 for IF); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Invitrogen, MA5-27912, 1:1000 for WB); β-tubulin (Cell Signaling Technology, 2128S, 1:200 for IF); ubiquitin (Cell Signaling Technology, 58395S, 1:1000 for WB); K48-linkage–specific antibody (Cell Signaling Technology, 8081S, 1:1000 for WB); ITCH (Santa Cruz, sc-28367, 1:1000 for WB, 1:50 for IF); ITCH (Novus Biologicals, NB100-68142, 1:50 for IHC); ITCH (Proteintech, 20920-1-AP, 1:1000 for WB, 1:50 for IHC); Tom20 (Santa Cruz, sc-17764, 1:50 for IF); Rab5 (Cell Signaling Technology, 3547T, 1:200 for IF); calnexin (Cell Signaling Technology, 2433S, 1:200 for IF); GM130 (Proteintech, 11308-1-AP, 1:1000 for WB, 1:200 for IF); TGN46 (Proteintech, 13573-1-AP, 1:1000 for WB, 1:200 for IF); Golgi-97 (Thermo Fisher Scientific, 14-9767-80, 1:200 for IF); TMEM192 (Santa Cruz, sc-518207, 1:1000 for WB); Myc (Abcam, ab32, 1:200 for IF); Myc (Proteintech, 60003-2-Ig, 1:1000 for WB); HA (MiliporeSigma, H6908, 1:1000 for WB, 1:200 for IF); HA (Santa Cruz, sc-7392, 1:1000 for WB); SOD1 (Enzo Life Sciences, ADI-SOD-100-F, 1:1000 for WB); USP9x (Santa Cruz, sc-365353, 1:1000 for WB); USP10 (Cell Signaling Technology, 8501T, 1:1000 for WB); USP7 (Santa Cruz, sc-137008, 1:1000 for WB); BRCC36 (Santa Cruz, sc-517392, 1:1000 for WB); USP11 (Santa Cruz, sc-365528, 1:1000 for WB); USP11 (Proteintech, 10244-1-AP, 1:50 for IF); USP15 (Proteintech, 14354-1-AP, 1:1000 for WB); USP24 (Proteintech, 13126-1-AP, 1:1000 for WB); GST (Santa Cruz, sc-138, 1:1000 for WB); IgG rabbit (MiliporeSigma, 12-370); IgG mouse (MiliporeSigma, 12-371); LAMP1 (Cell Signaling Technology, 9091S, 1:200 for IF); LC3B (Proteintech,14600-1-AP, 1:200 for IF); LC3B (Cell Signaling Technology, 3868S, 1:1000 for WB, 1:200 for IF); cathepsin L (Proteintech, 10938-1-AP, 1:1000 for WB); CTSB (R&D Systems, AF953, 1:1000 for WB); CTSB (Cell Signaling Technology, 31718T, 1:1000 for WB); cathepsin D (Cell Signaling Technology, 2284S, 1:1000 for WB); α-tubulin (Cell Signaling Technology, 3873, 1:1000 for WB); M6P (ABCD Antibodies, ABCD_AG949, 1:1000 for WB); and βIII-tubulin (MilliporeSigma, AB9354, 1:200 for IF).

    Techniques: Western Blot, Transduction, Inhibition, Binding Assay, Purification, Control, Immunoprecipitation

    ( A and B ) Lysates from HEK293 cells expressing Flag-tagged ITCH with various USPs were subjected to denaturing Flag immunoprecipitation. Immunoblot analysis of the precipitates using antibodies against ubiquitin (Ub) (A) or Ub-K48 (B) indicated that USP11, but not the other USPs, exhibited the ability to deubiquitinate ITCH ( n = 3). ( C and D ) Immunoblot analysis of the precipitates from denatured lysates of HEK293 cells expressing ITCH and USP11 or an inactivated form of USP11 (USP11-CS or U11-CS) was performed using antibodies against ubiquitin (C) or Ub-K48 (D). Wild-type USP11, but not the inactive USP11, had the capacity to remove the polyubiquitin chains from ITCH ( n = 3). ( E ) A cycloheximide chase assay indicated that the half-life of ITCH protein was shortened upon the knockdown of USP11 ( n = 3; * overall P < 0.05 for all time points, except for 0 hours, one-way ANOVA test). ( F ) A cycloheximide chase assay indicated that the half-life of ITCH protein was prolonged by wild-type USP11 but not the inactive USP11-CS, in the presence of rapamycin ( n = 3; * overall P < 0.05 for all time points, except for 0 and 4 hours, one-way ANOVA test). ( G and H ) IF analysis of the Golgi apparatus (GM130) in either wild-type (WT) or ITCH-knockout RPE1 cells expressing USP11 or USP11-CS. The confidence interval for wild-type RPE1 cells transfected with the empty vector or inactive USP11 is 0.75 to 1, whereas for cells transfected with wild-type USP11, the confidence interval is 0.5 to 0.75 (G). In ITCH-deficient RPE1 cells under different experimental groups, the confidence intervals are all 0.75 to 1. Scale bars, 5 μm. ( I and J ) IF analysis of the Golgi apparatus in RPE1 cells treated with either DMSO or 5 μM MTX for 24 hours. A significant increase in the number of cells with a more compact Golgi structure (both cis-Golgi: GM130 and trans-Golgi: TGN46) following MTX treatment. Scale bars, 5 μm.

    Journal: Science Advances

    Article Title: ITCH regulates Golgi integrity and proteotoxicity in neurodegeneration

    doi: 10.1126/sciadv.ado4330

    Figure Lengend Snippet: ( A and B ) Lysates from HEK293 cells expressing Flag-tagged ITCH with various USPs were subjected to denaturing Flag immunoprecipitation. Immunoblot analysis of the precipitates using antibodies against ubiquitin (Ub) (A) or Ub-K48 (B) indicated that USP11, but not the other USPs, exhibited the ability to deubiquitinate ITCH ( n = 3). ( C and D ) Immunoblot analysis of the precipitates from denatured lysates of HEK293 cells expressing ITCH and USP11 or an inactivated form of USP11 (USP11-CS or U11-CS) was performed using antibodies against ubiquitin (C) or Ub-K48 (D). Wild-type USP11, but not the inactive USP11, had the capacity to remove the polyubiquitin chains from ITCH ( n = 3). ( E ) A cycloheximide chase assay indicated that the half-life of ITCH protein was shortened upon the knockdown of USP11 ( n = 3; * overall P < 0.05 for all time points, except for 0 hours, one-way ANOVA test). ( F ) A cycloheximide chase assay indicated that the half-life of ITCH protein was prolonged by wild-type USP11 but not the inactive USP11-CS, in the presence of rapamycin ( n = 3; * overall P < 0.05 for all time points, except for 0 and 4 hours, one-way ANOVA test). ( G and H ) IF analysis of the Golgi apparatus (GM130) in either wild-type (WT) or ITCH-knockout RPE1 cells expressing USP11 or USP11-CS. The confidence interval for wild-type RPE1 cells transfected with the empty vector or inactive USP11 is 0.75 to 1, whereas for cells transfected with wild-type USP11, the confidence interval is 0.5 to 0.75 (G). In ITCH-deficient RPE1 cells under different experimental groups, the confidence intervals are all 0.75 to 1. Scale bars, 5 μm. ( I and J ) IF analysis of the Golgi apparatus in RPE1 cells treated with either DMSO or 5 μM MTX for 24 hours. A significant increase in the number of cells with a more compact Golgi structure (both cis-Golgi: GM130 and trans-Golgi: TGN46) following MTX treatment. Scale bars, 5 μm.

    Article Snippet: The following antibodies were used for WB, IF, and IHC with the indicated dilutions: Flag (Sigma-Aldrich, F1804, 1:200 for IF); Flag (Sigma-Aldrich, F3165, 1:1000 for WB); Flag (Cell Signaling Technology, 14793S, 1:400 for IF); glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Invitrogen, MA5-27912, 1:1000 for WB); β-tubulin (Cell Signaling Technology, 2128S, 1:200 for IF); ubiquitin (Cell Signaling Technology, 58395S, 1:1000 for WB); K48-linkage–specific antibody (Cell Signaling Technology, 8081S, 1:1000 for WB); ITCH (Santa Cruz, sc-28367, 1:1000 for WB, 1:50 for IF); ITCH (Novus Biologicals, NB100-68142, 1:50 for IHC); ITCH (Proteintech, 20920-1-AP, 1:1000 for WB, 1:50 for IHC); Tom20 (Santa Cruz, sc-17764, 1:50 for IF); Rab5 (Cell Signaling Technology, 3547T, 1:200 for IF); calnexin (Cell Signaling Technology, 2433S, 1:200 for IF); GM130 (Proteintech, 11308-1-AP, 1:1000 for WB, 1:200 for IF); TGN46 (Proteintech, 13573-1-AP, 1:1000 for WB, 1:200 for IF); Golgi-97 (Thermo Fisher Scientific, 14-9767-80, 1:200 for IF); TMEM192 (Santa Cruz, sc-518207, 1:1000 for WB); Myc (Abcam, ab32, 1:200 for IF); Myc (Proteintech, 60003-2-Ig, 1:1000 for WB); HA (MiliporeSigma, H6908, 1:1000 for WB, 1:200 for IF); HA (Santa Cruz, sc-7392, 1:1000 for WB); SOD1 (Enzo Life Sciences, ADI-SOD-100-F, 1:1000 for WB); USP9x (Santa Cruz, sc-365353, 1:1000 for WB); USP10 (Cell Signaling Technology, 8501T, 1:1000 for WB); USP7 (Santa Cruz, sc-137008, 1:1000 for WB); BRCC36 (Santa Cruz, sc-517392, 1:1000 for WB); USP11 (Santa Cruz, sc-365528, 1:1000 for WB); USP11 (Proteintech, 10244-1-AP, 1:50 for IF); USP15 (Proteintech, 14354-1-AP, 1:1000 for WB); USP24 (Proteintech, 13126-1-AP, 1:1000 for WB); GST (Santa Cruz, sc-138, 1:1000 for WB); IgG rabbit (MiliporeSigma, 12-370); IgG mouse (MiliporeSigma, 12-371); LAMP1 (Cell Signaling Technology, 9091S, 1:200 for IF); LC3B (Proteintech,14600-1-AP, 1:200 for IF); LC3B (Cell Signaling Technology, 3868S, 1:1000 for WB, 1:200 for IF); cathepsin L (Proteintech, 10938-1-AP, 1:1000 for WB); CTSB (R&D Systems, AF953, 1:1000 for WB); CTSB (Cell Signaling Technology, 31718T, 1:1000 for WB); cathepsin D (Cell Signaling Technology, 2284S, 1:1000 for WB); α-tubulin (Cell Signaling Technology, 3873, 1:1000 for WB); M6P (ABCD Antibodies, ABCD_AG949, 1:1000 for WB); and βIII-tubulin (MilliporeSigma, AB9354, 1:200 for IF).

    Techniques: Expressing, Immunoprecipitation, Western Blot, Ubiquitin Proteomics, Knockdown, Knock-Out, Transfection, Plasmid Preparation